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Phylogenetic Analysis of Porcine Circovirus 3 Circulating in Canadian Pigs

Abstract: Phylogenetic analysis of porcine circovirus 3 circulating in Canadian pigs

Introduction: Porcine circovirus 3 (PCV3) has been detected in pigs worldwide and associated with several clinical signs.
Methods: To investigate the genetic diversity of PCV3 strains circulating in Canada, 44 PCV3 positive samples from Saskatchewan (2/44), Manitoba (2/44), Quebec (4/44), Alberta (11/44) and Ontario (25/44) submitted to diagnostic laboratories in Canada between 2019 and 2021 were sequenced and analyzed.
Results: Phylogenetic analysis of capsid genes showed that all of the 44 Canadian strains classified into PCV3a and segregated into seven lineages with common amino acid changes observed at A24V, R27K, N56D, T77S, Q98R, L150I (F) and R168K positions.

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Porcine Gut Models in Nutrition Research - Dr. Li-Hsuan Chen

Video: Porcine Gut Models in Nutrition Research - Dr. Li-Hsuan Chen



In this episode of The Swine Nutrition Blackbelt Podcast, Dr. Li-Hsuan Chen, postdoc researcher at Aarhus University, discusses how porcine intestinal cell lines and organoids can help study the impact of nutrition on gut health. He explains how in vivo piglet trials identify diet-driven shifts in microbiota and metabolites, while in vitro models allow researchers to test specific metabolites, barrier function, immune markers, and future organ-on-chip systems before moving back to animal studies. Listen now on all major platforms!

"In vitro models can reduce early animal use by helping researchers screen nutritional candidates before moving back to in vivo trials."

Meet the guest: Dr. Li-Hsuan Chen / li-hsuan-chen-9a95a7279 is a postdoc researcher at Aarhus University in Denmark. Research focuses on porcine gut health, host-microbe interactions, cell lines, and organoids, with PhD work at Aarhus University studying how dietary protein and amino acid levels shape gut microbiota, microbial metabolites, and intestinal epithelial responses. Learn more from Dr. Li-Hsuan Chen on The Swine Nutrition Blackbelt Podcast, available on all major platforms.