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Replication of Porcine Deltacoronavirus Is Limited in the Gastrointestinal Tract of Neonatal Piglets Co-infected Simultaneously or 16 Hours Prior With Virulent Porcine Epidemic Diarrhea Virus

Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) cause acute diarrhea/vomiting in neonatal pigs and share similar tissue or cellular tropisms in the gastrointestinal tract. We investigated if or how these two swine enteric coronaviruses interact with each other in gnotobiotic (Gn) piglets. Seventeen 9-10-day-old Gn piglets were randomly assigned to 5 groups and inoculated with PEDV strain PC21A [9.3 log10 genomic equivalents (GE)/pig] and/or PDCoV strain OH-FD22 (8.6 log10 GE/pig) as follows: dually with PEDV and PDCoV [16 h later (n = 4) or simultaneously (n = 3)] or singly with PEDV (n = 4), PDCoV (n = 4), or mock (n = 3). No enhanced clinical disease or fecal PEDV shedding were observed in dually inoculated pigs compared with PEDV or PDCoV singly inoculated pigs, coinciding with no significant differences in jejunal VH:CD ratios and PEDV antigen-positive scores at post-inoculation days (PIDs) 3-4 among the groups. These observations indicate no increased severity of PEDV infectivity by PDCoV co-infection. Notably, compared with PDCoV singly inoculated pigs, low to moderate fecal PDCoV RNA titers were detected only at PID 1 in both dually inoculated pig groups. At PIDs 2-4, however, there was no detectable PDCoV RNA in the feces, coinciding with no or few PDCoV antigen-positive cells in the small and large intestine of the dually inoculated pigs at PIDs 3-4. These observations indicate a possible interference or inhibition of PDCoV replication in the gastrointestinal tract of pigs co-infected with PEDV and may influence PDCoV infection in PEDV co-infected pigs.

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Sheep Health Boost: How We Keep Our Flock Healthy At Ewetopia Farms With De-licing And Deworming

Video: Sheep Health Boost: How We Keep Our Flock Healthy At Ewetopia Farms With De-licing And Deworming

Welcome back to Ewetopia Farms! In today’s exciting episode of our daily sheep farming vlog, we're diving deep into essential health management practices for our flock. Join us as we not only give our Suffolk and Dorset sheep their crucial booster doses of de-lice medication but also deworm our ram lambs with a specialized treatment that targets common sheep parasites, including tapeworms.