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MATE Transporter Facilitates Bitter Saponin Transport From Cytoplasm to Vacuole

By Zhang Nannan

Domestic soybeans serve as an important source of healthy edible plant oil and high-quality plant protein in our food culture. In addition to the well-known components of oil (approximately 20%), protein (approximately 40%), and carbohydrates (approximately 30%), soybean seeds also contain a range of specialized metabolites that promote human health. For example, soyasaponins, which make up 0.5–6.5% of the seed, and isoflavonoids, which make up 0.01–0.5%, are critical for promoting human health.

A research team led by Dr. Wang Guodong from Institute of Genetics and Developmental Biology of the Chinese Academy of Sciences (CAS) used gene co-expression analysis to identify GmMATE100 as a  for soyasaponin transport among 117 members of the Multidrug and Toxic Compound Extrusion (MATE) transporter family in the soybean genome.

The study, titled "GmMATE100 is involved in the import of soyasaponins A and B into vacuoles in soybean plants (Glycine max L.)," was published in the Journal of Agricultural and Food Chemistry on April 22.

Subcellular localization experiments showed that GmMATE100 is localized to the vacuolar membrane in both plant and . In vitro yeast transport assays revealed that GmMATE100 has activity in transporting both type A and type B soyasaponins, but is unable to transport the soyasaponin precursor, soyasapogenol.

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Why Seed Analysts are Thriving Under Seeds Canada

Video: Why Seed Analysts are Thriving Under Seeds Canada

Last month in Edmonton, Alta., industry leaders and stakeholders gathered to discuss the evolving landscape of the seed industry at Seeds Canada’s annual conference. Among them was Sarah Foster, president of 2020 Seed Labs and the new vice-president of Seeds Canada.

Foster, who has been on the board of Seeds Canada for over a year, has witnessed firsthand the challenges and opportunities that come with the formation of the organization. Seeds Canada was established just over three years ago through the merger of multiple seed industry groups, including the Commercial Seed Analysts Association of Canada (CSAAC). Since then, the organization has been working to define its priorities and solidify its role in the industry.

“The challenge has been allowing the dust to settle after the merger,” Foster explained. “We’ve been focused on identifying what our priorities should be and ensuring that our members, especially the seed analysts, are getting what they need to continue their professional work.”

One of the recent highlights was a pre-conference event where the three major seed labs in Alberta — 20/20 Seed Labs, SGS Canada and Seed Check — opened their doors to members. The event saw a record number of seed analysts and business professionals in attendance. Foster emphasized the importance of this transparency, stating, “It’s crucial for people to see what goes on behind the scenes. We’re an open book now, and that openness helps build trust and understanding within the industry.”

The event also featured an environmental scan and a series of discussions that fostered strong communication among attendees. According to Foster, the dialogue was both encouraging and inspiring.

“A lot of people were really inspired by the fact that Seeds Canada is moving ahead with its agenda. The seed analysts, who have always worked diligently in the background, are now being recognized more prominently,” she said.

Before the merger, seed analysts were represented by CSAAC. Now, as part of Seeds Canada, they are finding their place within the larger organization. Foster believes that the integration has been successful, noting, “I think we’re thriving. You only need to look south of the border, where similar consolidations are happening.”

As Seeds Canada continues to evolve, Foster remains optimistic about the future. “I want to be totally transparent with anyone who is a seed analyst — I’ve got your back. We’re moving in a positive direction, and we’ll do everything we can to meet the needs of our members,” she said.